Osasuna's appeal over Barcelona defender Inigo Martinez's eligibility to play against them has been dismissed.
Martinez did not join up with the Spain squad for the recent international break because of a knee injury.
Fifa rules prohibit a player from playing for his club within five days - unless he has the national FA's permission - if he has not linked up with his country when called up.
Barca's 3-0 win over Osasuna in La Liga, rearranged from 8 March after the death of club doctor Carles Minarro Garcia, came the day after the international window ended - and four days after Spain's Nations League match with the Netherlands.
Martinez played the full 90 minutes against Osasuna on 27 March and the Basque club launched an appeal to the Royal Spanish Football Federation (RFEF) a day later.
However, the RFEF's competition committee deemed there was no infringement by Barcelona, as the player's withdrawal process was carried out correctly and in accordance with the regulations.
Osasuna plan to file a second appeal with the RFEF's appeal committee.
Barcelona are four points clear at the top of La Liga while Osasuna are 13th.
