New head coach Charlotte Edwards will make England players "accountable" for their fitness following criticism during a dismal winter.
England were eliminated in the group stage at the T20 World Cup, then humiliated 16-0 in the Ashes in Australia.
Head coach Jon Lewis and captain Heather Knight were both sacked last month, with legendary former skipper Edwards confirmed as Lewis' replacement on Tuesday.
"There is fitness testing next week and I'll judge for myself where they are at," Edwards told BBC Sport. "I don't want people talking about the fitness of the England women's cricket team. Fitness is a non-negotiable. We should be fit."
After England's early exit from the T20 World Cup in October, former spinner Alex Hartley said some players were "letting the team down" in terms of fitness.
Athleticism again came into focus during the Ashes clean sweep, but Lewis attributed that to a cultural difference between the UK and Australia.
The Lewis regime was also characterised by a more relaxed approach. During the World Cup, some England players attended boat parties in Dubai.
"Honesty and hard work are key to any culture," said Edwards, England's all-time leading run-scorer.
"If we've got that in place we will move pretty quickly in the right direction. We've got to work hard. That's for me to implement in training. We will talk to our science and medicine teams in terms of what we have to do with our fitness standards."
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Edwards won the Ashes and both World Cups as England captain only to be sacked in 2016 and replaced by Knight.
Now Knight has been sacked but has indicated her desire to continue as a player. Edwards, 45, said she was not consulted in the decision to remove Knight.
"She sets such high standards, she's the ultimate professional and what we need around the England team right now," said Edwards.
"She's playing as well as I've ever seen her play. It was hard to watch the Ashes, because of the toll it was taking on her. Without the captaincy, hopefully she'll be able to go on to bigger and better things as a player."
In order to take the England job, Edwards has left all of her roles in domestic cricket, including her post as Mumbai Indians head coach in the Women's Premier League. While he was England coach, Lewis was in charge of UP Warriorz in the same competition.
England will appoint a new national selector, as well as a new captain, which Edwards said will be in place for the series against West Indies in May.
Knight's vice-captain Nat Sciver-Brunt has previously said she would be interested in the job, ...
